Regulated degradation of hmg coa reductase requires conformational changes in sterol-sensing domain

HIGHLIGHTS

• who: Hongwen Chen from the Generation of Fab , Monoclonal antibody (designated , ) of isotype Ig, were raised in mice (NZBWF1/J, The Jackson Laboratory) at the Department of Molecular Genetics of the University of Texas Southwestern Medical CenterThe antigen immunize mice was the HMGCRTM-UBIAD N S complex that was reconstituted into Amphipol, . Hybridomas were created by fusion of splenic B lymphocytes from hyperimmune mice to SP2-m, mouse myeloma cells (ATCC, CRL-2016). We used the combined techniques of ELISA, immunoblot analysis, and immunoprecipitation to identify antibodies that preferentially bound to HMGCRTMs-UBIAD N S complex in . . .
  

   

  

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