Regulation of rhogef activity by intramolecular and intermolecular sh3 domain interactions*

HIGHLIGHTS

  • who: Martin R. Schiller from the (UNIVERSITY) have published the paper: Regulation of RhoGEF Activity by Intramolecular and Intermolecular SH3 Domain Interactions*, in the Journal: (JOURNAL) of November/21,/2005
  • what: The authors examined the ability of intramolecular SH3 domain/ PXXP interactions to contribute to the regulation of GEF activity.
  • how: The authors examined whether the intramolecular SH3 domain/PKTP interaction modulates the activity of Kal-GEF1 toward Rac1 in fluorogenic GEF assays with purified fragments of Kalirin (Fig 5A) . Similar results were obtained using GSTRhoG as a substrate (data not shown).
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