HIGHLIGHTS
- who: Yuqing Li from the Developing seeds at, and , days after flowering (DAF) of Bnapus accessions , (high oil content) and , (low oil content) were collected from the field under natural conditions (three replicates for each line). The detail information of the oil content and fatty acid composition of , and , seeds were described previously [34]. Total RNA was extracted from the seeds using TRIzol reagent (Invitrogen, USA) and treated with DNase (Promega, Madison, WI) according to the manufacturer`s instructions. Total RNA samples were then tested by (1), % agarose electrophoresis for degradation and impurities, (2) Keo , spectrophotometer for . . .
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