Regulatory chromatin rewiring promotes metabolic switching during adaptation to oncogenic receptor tyrosine kinase inhibition

HIGHLIGHTS

• who: Samuel Ogden from the For cell cycle analysis, media was collected from cells and cells were dissociated using trypsin, using the collected media to quench trypsinCells were collected by centrifugation, washed with PBS and then fixed by the addition of, % ethanol, pre-cooled to , u00b0C. Fixed cells were then stored at , u00b0C until required. Cells were then pelleted and washed with PBS before the addition of , u03bcL , u03bcg/mL propidium iodide (Sigma, ). Cells were then analysed by the University of Manchester Flow Cytometry Core Facility on a BD Biosciences LSRFortessaTM. Data was analysed using ModFit LTTM . . .
  

   

  

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