Relating cyp2b6 genotype and efavirenz resistance among post-partum women living with hiv with high viremia in uganda: a nested cross-sectional study

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  • who: Allan Buzibye from the Drug resistance testing was performed at the Infectious Diseases Institute Core Laboratory, Kampala, Uganda, which is accredited by the College of American Pathologists (CAP)Viral RNA was isolated from plasma samples using the Invitrogen Purelink viral RNA/DNA kit (Thermo Fisher Scientific, Dreieich, Germany). , genotyping was performed on extracted RNA using an in-house , genotyping assay covering the protease (PR) and reverse transcriptase (RT) with subsequent sequencing using the BigDye Terminator v3., Cycle Sequencing Kit (Thermo Fisher Scientific, Dreieich, Germany). Sequence analysis was performed using , capillary sequencer (Thermo Fisher Scientific, Dreieich, Germany . . .

     

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