Relaxin activates peroxisome proliferator-activated receptor γ (pparγ) through a pathway involving pparγ coactivator 1α (pgc1α)*

HIGHLIGHTS

• who: Sudhir Singh from the EXPERIMENTAL PROCEDURES Cells and Transfections-The HEK-, cells are HEK T cells stably expressing , described previously (12)For reporter assays, the HEK-, cells were transfected using Fugene, (Roche) as described previously (12). The plasmids included ACO-PPRE-luc (24) provided by Brian Seed (Harvard University), PGC1␣-promoter luciferase reporter (25) and plasmids encoding PKI and inactive mutant (PKImut) (26) from Addgene, dominant-negative CREB (KCREB, Clontech, Mountain View, CA), cAMP response element reporter(CRE-luc), and Renilla luciferase plasmid (pTK-RL) (all from Promega). For cotransfection of siRNA with reporter plasmids, Dharmafect . . .
  

   

  

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