HIGHLIGHTS
- who: Ganesh Agam from the (UNIVERSITY) have published the paper: Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins, in the Journal: (JOURNAL) of 31/01/2023
- what: The work demonstrates the ability of smFRET experiments to simultaneously measure distances and avoid the averaging of conformational dynamics for realistic protein systems, highlighting its importance in the expanding toolbox of integrative structural biology. Based on the estimated distance uncertainty, the authors propose a threshold of u0394Rapp(u03ba2) and amp;lt; 10% to identify measurements with dye-related artifacts . . .
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