Repair of oxidized proteins

HIGHLIGHTS

• who: Re̕gis Grimaud from the Chemicals and Growth Conditions-Unless noted otherwise, chemicals were purchased from SigmaThioredoxin reductase was a gift from Dr. C. Williams (University of Michigan, Ann Arbor, MI). Bacteria were grown on Luria-Bertani medium or , minimal medium. Plasmids-DNA manipulations were carried out using standard techniques, according to the manufacturer's instructions. Plasmid pET MsrA was obtained by cloning the msrA gene in the pET21a vector (Novagen). Expression from this vector results in the synthesis of a protein with a His tag on the C terminus. The msrA coding region was amplified . . .
  

   

  

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