Replacing sanger with next generation sequencing to improve coverage and quality of reference dna barcodes for plants

HIGHLIGHTS

• who: Mike J. Wilkinson from the Plant materials and DNA extractionPlant samples were collected from the Murraylands region of South Australia during February-March , and October , (Supplementary Table, ). Specimens were identified by reference to the Flora of South Australia47, and Atlas of Living Australia (www.ala. org.au). Herbarium specimens were generated and lodged with the State Herbarium of South Australia. Diversity of the sample set was increased by the addition of nine Gymnosperm species (for which rbcL and matK data were available in BOLD) from the Waite Arboretum (University of Adelaide, South Australia). DNA was extracted . . .
  

   

  

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