Reporter systems for assessments of extracellular vesicle transfer

HIGHLIGHTS

• who: Gangjian Qin from the Department of Biomedical Engineering, School of Medicine, University of Alabama at Birmingham, Birmingham, AL, Birmingham, AL, United States have published the article: Reporter Systems for Assessments of Extracellular Vesicle Transfer, in the Journal: (JOURNAL)

SUMMARY

Among different bioluminescence-generating enzymes, it appears that NanoLuc or ThermoLuc, when tethered to CD63, are preferred for sensitive imaging and tracking of EVs in_vivo and in_vitro. Besides CD63, lactadherin was also engineered, such as by fusion with Gaussia luciferase to visualize and track exosomes in_vivo. In a sophisticated system, EV surface was . . .

 



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