Resveratrol rescues cutaneous radiation-induced dna damage via a novel ampk/sirt7/hmgb1 regulatory axis

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SUMMARY

Functional studies using in_vitro Official journal of CDDpress 2 and in_vivo models revealed that RSV with defined concentration acts as a promising radioprotective agent to alleviate IR-induced DNA damage and cell death in keratinocytes and cutaneous tissues. To detect whether RSV directly activates SIRT7, equal amounts of purified SIRT7 protein from in_vitro-expression were incubated with RSV or DMSO in reaction buffer in each well. The SIRT7 and HMGB1-GFP expressing constructs for mammalian cell expression and SIRT7-GST construct for in_vitro E.coli expression and following purification was purchased from YouBio Biological . . .

 



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