Reverting tp53 mutation in breast cancer cells: prime editing workflow and technical considerations

HIGHLIGHTS

  • who: Asmaa Y. Abuhamad and collaborators from the University Putra Malaysia, Serdang, Selangor, Malaysia have published the article: Reverting TP53 Mutation in Breast Cancer Cells: Prime Editing Workflow and Technical Considerations, in the Journal: Cells 2022, 11, 1612. of 10/04/2022
  • what: The authors aimed to assess the efficiency and establish a prime editing workflow by reverting a TP53 missense mutation in the T47D luminal A breast cancer cell line (c.580 C > T, p.L194F). The authors aimed to introduce the C > T mutation at the position c.580 of the TP53 coding_sequence . . .

     

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