Review of methods for the detection of allergens in novel food alternative proteins

HIGHLIGHTS

• What: This approach analyses the data from allergic individuals to statistically calculate threshold doses of allergen able to elicit a reaction in a given proportion of them (shrimp allergic subjects in this case). The allergen investigation in this study aimed to determine if tropomyosin, arginine kinase and myosin (three main crustacean cross-reactive allergenic proteins) were identified in BSF, and whether the feed and method of killing had an influence on the allergens detected. This study showed that silkworm tropomyosin shares 73-92.3% sequence homology with other allergenic tropomyosins (by BLAST bioinformatics amino_acid sequence alignment). In . . .
  

   

  

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