Rna sequencing and de novo assembly of the digestive gland transcriptome in mytilus galloprovincialis fed with toxinogenic and non-toxic strains of alexandrium minutum

HIGHLIGHTS

  • who: Mytilus galloprovincialis and collaborators from the cDNA libraries were prepared and subjected to massive sequencing at the Biotechnology Center of the University of Illinois, using an Illumina GAII sequencing platform and a , bp paired-end sequencing strategyThe output sequencing reads were further processed for adapter removal and trimming, according to the base calling quality. The resulting sequences were assembled with Trinity using the default options and a minimum allowed length of , bp [83]. The overall quality of the assembly was improved with the addition of, , Illumina reads obtained from the DG of naive mussels (unpublished data . . .

     

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