Role of human adipose-derived stem cells (hadsc) on tgf-β1, type i collagen, and fibrosis degree in bladder obstruction model of wistar rats

HIGHLIGHTS

• who: Safendra Siregar from the paraffin blocksParaffin-embedded tissue was sliced and placed on an object-glass, followed by deparaffinization and rehydration. Hydrogen peroxide was administered for , min to eliminate endogenous peroxidase activity. The sample was then rinsed using PBS-Triton X , (Tx). Hematoxylin-eosin (HE) staining was performed to assess structural changes in the bladder tissue. To determine smooth muscle and collagen, cross-sections were stained with Masson`s trichrome (MT) using the MT staining kit. Smooth muscle areas (red stains) and collagen (blue-green stains) were evaluated in, u00d7 magnification images of tissue using a . . .
  

   

  

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