Role of ww domain-containing oxidoreductase wwox in driving t cell acute lymphoblastic leukemia maturation*

HIGHLIGHTS

• who: Shenq-Shyang Huang from the nor and acceptor fluorescent molecules, respectivelyThe FRET images were corrected for background fluorescence from an area without cells and spectral bleed-through. The spectrally corrected FRET concentration (FRETc) was calculated by Youvan's equation (using a software program Image Pro, .1, Media Cybernetics): FRETc ⫽ (fret ⫺ bk[fret] ⫺ cf[don] ⫻ (don ⫺ bk[don] ⫺ cf[acc] ⫻ (acc-bk[acc]), where fret ⫽ fret image, bk ⫽ background, cf ⫽ correction factor, don ⫽ donor image, and acc ⫽ acceptor image. The equation normalizes the FRET signals to the expression levels of the proteins. Where indicated, time-lapse microscopy . . .
  

   

  

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