Rufy1 binds arl8b and mediates endosome-to-tgn ci-m6pr retrieval for cargo sorting to lysosomes

HIGHLIGHTS

• who: RUFY and colleagues from the Immuno-electron microscopy Cells were fixed in, % PFA (Electron Microscopy Sciences) and, % Glutaraldehyde (Sigma-Aldrich) in, ., M Phosphate Buffer (PB) for , h at RT. The fixative was replaced with, % PFA in, ., M PB, and samples were stored until further processing as described before (Slot and Geuze, ). Briefly, samples were rinsed in PBS, blocked with, .15% glycine in PBS, scraped in, % gelatin in PBS, pelleted, and embedded in, % gelatin. Small blocks of the pellet were cryoprotected with, ., M sucrose, mounted on aluminum pins and plunge frozen in liquid nitrogen. Ultrathin cryosections were . . .
  

   

  

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