Semi-site-specific primer pcr: a simple but reliable genome-walking tool

HIGHLIGHTS

• who: Cheng Wei and collaborators from the State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China have published the research: Semi-Site-Specific Primer PCR: A Simple but Reliable Genome-Walking Tool, in the Journal: (JOURNAL)
• what: In this work, a new genome-walking approach, termed semi-site-specific primer PCR (3SP-PCR), is proposed for the rapid amplification of unknown DNA sequences adjacent to a known region.

SUMMARY

Due to the use of a sitespecific primer pair, the amplification specificity of inverse PCR is high. This target . . .

 



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