Separate roles for the golgi apparatus and lysosomes in the sequestration of drugs in the multidrug-resistant human leukemic cell line hl-60*

HIGHLIGHTS

• who: Yuping Gong from the The human acute promyeloid leukemia cell line , and its doxorubicin-selected resistant , ADR cell line were kindly provided by DrYueshang Zhang (Arizona Cancer Center, University of Arizona). Cell lines were grown in RPMI , medium supplemented with, % fetal calf serum, mM Hepes, mM sodium pyruvate, .1% penicillin, .1% streptomycin. Cells were maintained at a density of , ⫻ , to , ⫻ , cells/ml at , °C in a humidified, % , atmosphere. Daunorubicin, MK571, nigericin, monensin, concanamycin A, buthionine sulfoximine (BSO) were purchased from Sigma. Sulforhodamine, NBDC6-ceramide, FITC-dextran (molecular weight, , ), (5-and, ) carboxyl , AM acetate ester, Lysotracker Green . . .
  

   

  

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