Sequential proteolytic processing of the capsular caf1 antigen of yersinia pestis for major histocompatibility complex class ii-restricted presentation to t lymphocytes*

HIGHLIGHTS

• who: Julie A. Musson from the EXPERIMENTAL PROCEDURES Antigens and Cells-Recombinant Caf, protein (rCaf1) of Ypestis was cloned and expressed in Escherichia coli and purified as described previously (26). Denatured rCaf, was prepared by boiling rCaf, for , min, cooling, and using immediately as described previously (26). Synthetic peptides of , amino acids in length based on the published Caf, sequence (accession number Q AJ6) were synthesized by Dr J. Gray, Institute of Cell and Molecular Biosciences, University of Newcastle, UK. Peptides were shown to be non-mitogenic and non-toxic to proliferating T cells. Culture media, metabolic . . .
  

   

  

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