HIGHLIGHTS
SUMMARY
Microbial components in the eye are known to stimulate toll-like receptors on the ocular surface epithelial_cells, thereby activating the ocular surface immune system. To understand effect of the microbes to ocular surface inflammation, the impact of preponderant microbial species on cytokine expression by ocular surface epithelial_cells was investigated. Human corneal epithelial_cells (H-CET) were cultured to near confluency in DMEM/F12 medium containing 5% FBS (Life Technology, Singapore) media, with passaging and maintenance of culture conditions as described in previous studies. Addition of A. xyloxidans to corneal epithelial_cells led to increased inflammatory cytokine . . .
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