Single-cell dna methylation sequencing by combinatorial indexing and enzymatic dna methylation conversion

HIGHLIGHTS

• who: Zac Chatterton from the NextSeq-Post-mortem flash-frozen prefrontal cortex tissue was obtained from a, year-old female donor with no diagnosis of neurological diseasePost-mortem flashfrozen cortex was obtained from a genetically modified (C9orf72) mouse. Following the protocols of Mulqueen et al [, ]. Brain tissue sections were resuspended in , mL of ice-cold NIB-HEPES solution (, mM HEPES, mM NaCl, mM M u00ad gCl2). The tissues were equilibrated (, min) and then dounce homogenized (, loose strokes and , tight strokes) and filtered through, u03bcm cell strainers (BD Biosciences, ). Nuclei were pelleted (, g) and were transferred to a . . .
  

   

  

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