Single-cell rna-seq integrated with multi-omics reveals serpine2 as a target for metastasis in advanced renal cell carcinoma

HIGHLIGHTS

• who: Wen-jin Chen from the Animals All animal experiments were approved by the Animal Care and Ethical Committee of the Second Military Medical University (Shanghai, China)The, week-old male nude mice were randomly injected with PBS , u03bcl containing , u00d7 , , SERPINE2-OE-PR-luc cells or, PR-luc via the tail vein. After, week injection, the mice were sacrificed and the lung tissue was removed and fixed in, % formalin buffer solution. , PR cells were transfected with luciferase reporter gene to detect subrenal tumor formation or lung metastasis. Tumor growth was monitored weekly using IVIS Lumina imaging . . .
  

   

  

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