Single-copy snail upregulation causes partial epithelial-mesenchymal transition in colon cancer cells

HIGHLIGHTS

• who: Fatima Junaid from the Cells were cultured in Dulbecco`s Modified Eagle Medium (Gibco) containing , g/L, Glucose and, Glutamine and supplemented with, % tetracyclinefree foetal bovine serum (Clontech) and, % penicillin and streptomycin (Gibco). Cells were passaged when confluent with, .05% trypsin (Gibco) and incubated in a Heracell , incubator, % oxygen and, % carbon dioxide at , u00b0C. , cells were a kind gift from Stephen Taylor, University of Manchester. HCT-116, SW480, and , cells were from group stocks. All cell lines were verified by short-tandem repeat genotyping undertaken by the inhouse Biorepository. Cell cultures were regularly tested for mycoplasma . . .
  

   

  

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