HIGHLIGHTS
- who: Ran Chai and collaborators from the College of Life Sciences, Henan Agricultural University, Key Laboratory of Enzyme Engineering of Agricultural Microbiology, Ministry of Agriculture and Rural Affairs, Zhengzhou, China have published the research: Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in Escherichia coli and Pseudomonas, in the Journal: Microorganisms 2023, 11, 850. of /2023/
- how: In this study SSB was used to replace the u03bb-Red recombination system in pCas and was confirmed to efficiently promote HR- and NHRmediated gene editing by the pCas/pTargetF system . . .
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