Site-directed mutagenesis of the calcium-binding site of blood coagulation factor xiiia*

HIGHLIGHTS

• who: Thung-S. Lai from the amine substrate (5-(biotinamido)pentylamine) was determined using an assay as described earlier (22-24)The transglutaminase activity data were transformed by an Eadie-Hofstee plot to determine the Km. The enzyme concentration used in the assays expressed, % of the total thrombin-independent FXIIIa activity. The measurement of the activation constant (Kact) for calcium ions was carried out by incubating FXIII with thrombin in the absence of calcium chloride at , °C for , min, followed by exposure to, mM calcium chloride, and then measuring the calcium-dependent activity by the H incorporation . . .
  

   

  

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