HIGHLIGHTS
SUMMARY
The previous studies highlighted the expression of some keratins in_vitro that were absent in resting condition in_vivo. The authors investigated K3 and K13 expression in_vivo and in_vitro. As expected, K13 was only detected in in_vivo samples of oral mucosa and conjunctiva, whereas in_vitro it was present only in large and differentiated cells of cultured limbo-corneal colonies. Due to the discrepancy between in_vivo and in_vitro expression, these two keratins cannot be reliable markers to identify the oral mucosal epithelium, specifically in a pathologic environment. Stem-cell-enriched holoclones derived from three in_vitro cultured tissues . . .
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