Spatial mapping of the total transcriptome by in situ polyadenylation

HIGHLIGHTS

  • who: David W. McKellar from the (UNIVERSITY) have published the article: Spatial mapping of the total transcriptome by in situ polyadenylation, in the Journal: (JOURNAL) of 28/04/2022
  • what: The authors demonstrate that enzymatic in situ polyadenylation of RNA enables detection of the full spectrum of RNAs, expanding the scope of sequencing-based spatial transcriptomics to the total transcriptome. The authors demonstrate that the spatial total RNA-sequencing (STRS) approach captures coding RNAs, noncoding RNAs and viral RNAs.
  • how: The authors investigated the utility and versatility of STRS by applying it to . . .

     

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