Spatiotemporal-resolved protein networks profiling with photoactivation dependent proximity labeling

HIGHLIGHTS

• who: Yansheng Zhai from the Plasmid construction Supplementary Table , lists the genetic constructs used in this studyThe miniSOG and KillerRed sequences were cloned from gift plasmids from P. Zou (Peking University). Mitochondrial matrix targeting sequence was derived from the, terminal, amino acids of COX4, then cloned into specified vectors by Gibson assembly (Beyotime, #D S). As for targeting to endoplasmic reticulum membrane and nucleus, human SEC B (NM_006808.3) DNA amplified via PCR (NEB, #M L) from HEK T cell cDNA library and H B DNA (a gift from D. Lin at Shenzhen Bay Laboratory) were used . . .
  

   

  

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