Spatiotemporally controlled generation of ntps for single-molecule studies

HIGHLIGHTS

  • who: Anton Sabantsev from the DepartmentUppsala University have published the Article: Spatiotemporally controlled generation of NTPs for single-molecule studies, in the Journal: (JOURNAL) of 11/02/2022
  • what: The experiments (e f) were carried out on a Nikon Eclipse Ti2-E motorized microscope equipped with a CFI Apochromat TIRF 60XC objective. a.u. arbitrary unit. __NEWPAGE__Articles NATuRE CHEMIcAL BIOLOGy uncaged ATP into neighboring regions.
  • how: To assess the reproducibility of LAGOON measurements the authors conducted helicase experiments on three FOVs in three different flow cells with ~1 W cm-2 360-nm . . .

     

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