Specific proteolysis of the kinase protein kinase c-related kinase 2 by caspase-3 during apoptosis

HIGHLIGHTS

• who: Vincent L. Cryns from the (UNIVERSITY) have published the paper: Specific Proteolysis of the Kinase Protein Kinase C-related Kinase 2 by Caspase-3 during Apoptosis, in the Journal: (JOURNAL)
• what: Using this approach, the authors isolated a partial cDNA encoding protein kinase C-related kinase 2 (PRK2), a serine-threonine kinase, and demonstrate that full-length human PRK2 is proteolyzed by caspase-3 at Asp117 and Asp700 in_vitro.

SUMMARY

Small cDNA pools (approximately 100 clones each) are transcribed/translated in_vitro in the presence of_[35S]methionine; these labeled protein . . .

 



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