Spectroscopic and kinetic studies of the reaction of bromopropanesulfonate with methyl-coenzyme m reductase*

HIGHLIGHTS

  • who: Ryan C. Kunz from the Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, have published the paper: Spectroscopic and Kinetic Studies of the Reaction of Bromopropanesulfonate with Methyl-coenzyme M Reductase*, in the Journal: (JOURNAL) of NOVEMBER/10,/2006
  • what: MCRred1 used for the experiments presented in Figs 2, 5, 8, 9, S1, and S2 was purified as described with the following modifications . In the studies described here, MCRred1 was reacted with BPS, and the propylsulfonate ligand was unambiguously identified (after acid quenching) as propanesulfonate by NMR spectroscopy and by HPLC analysis in both . . .

     

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