Speedingcars: accelerating the engineering of car t cells by signaling domain shuffling and single-cell sequencing

HIGHLIGHTS

• who: Rocu00edo Castellanos-Rueda from the Primary human T cell isolation and culture Buffy coats from healthy donors were obtained from the Blutspendezentrum (University of Basel) following the general consent guidelines approved by swissethics (Swiss Association of Research Ethics Committees)All recruited volunteers provided written informed consent. Peripheral blood mononuclear cells (PBMCs) were extracted from buffy coats using a Ficoll gradient. T cells were then isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human, activator CD3/, Dynabeads (Gibco) at a bead:cell ratio of, :1. Activated T cells were cultured in, VIVO . . .
  

   

  

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