Sphingosine kinase 1 is an intracellular effector of phosphatidic acid*

HIGHLIGHTS

• who: Christine Delon from the Plasmids-The wild type and the G D catalytically dead (KD) mouse , were cloned in pcDNA4/myc-His (Invitrogen)These and the, and, terminal , fragments (residues, and , respectively) were also sub-cloned into pEGFP-, (Clontech) using appropriate primers and standard techniques. , was cloned in the p, vector as described previously (19). Antibodies-The following antibodies were used in immunofluorescence and immunoblots: mouse monoclonal , against the myc epitope (prepared at Babraham) have published the paper: Sphingosine Kinase 1 Is an Intracellular Effector of Phosphatidic Acid*, in the Journal: (JOURNAL) of May/24,/2004 . . .
  

   

  

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