Stable integration of inducible splics reporters enables spatio-temporal analysis of multiple organelle contact sites upon modulation of cholesterol traffic

HIGHLIGHTS

• who: Flavia Giamogante and colleagues from the Department of Biomedical Sciences, University of Padova, Padova, Italy have published the research work: Stable Integration of Inducible SPLICS Reporters Enables Spatio-Temporal Analysis of Multiple Organelle Contact Sites upon Modulation of Cholesterol Traffic, in the Journal: Cells 2022, 1643 of /2022/
• what: The authors report here on the generation of a new vector library of cloned into a piggyBac system for stable and expression of the in a cell line of interest to overcome any potential weakness due to variable protein expression in transient transfection studies.
 



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