Stable overexpression and targeted gene deletion of the causative agent of ash dieback hymenoscyphus fraxineus

HIGHLIGHTS

  • who: Tobias Lutz from the Institute of Plant Science and Microbiology, Molecular Phytopathology, University of Hamburg, Ohnhorststr18, Hamburg, Germany have published the Article: Stable overexpression and targeted gene deletion of the causative agent of ash dieback Hymenoscyphus fraxineus, in the Journal: (JOURNAL)
  • what: The authors provide a protocol for of H. to obtain fluorescence reporter strains and targeted gene knock-out mutants.
  • how: Based on these results they concluded that these spermatia are potentially infectious. In the following paragraphs the final protocol for protoplasting for transformation is described. In preliminary experiments the 3 . . .

     

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