Stage-specific requirement for mettl3-dependent m6a modification during dental pulp stem cell differentiation

HIGHLIGHTS

  • who: Haiyun Luo from the Dental pulp tissues were collected according to the appropriate guidelines after written informed consent was obtained by a protocol approved by the Human Research Committee of Stomatological Hospital, Southern Medical University (ethical code, (16))Primary DPSCs were harvested and cultured as previously described [15]. Briefly, dental pulp tissues were removed from extracted third molars and then digested with , mg/ mL collagenase I (Gibco-Invitrogen, Carlsbad, CA, USA). DPSCs were collected and cultured in Dulbecco`s modified Eagle`s medium (DMEM) supplemented with, % fetal bovine serum and, % penicillin and streptomycin (all from Gibco . . .

     

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