Staining the endoplasmic reticulum in combination with antibody staining

HIGHLIGHTS

  • who: ReZolve-ER et al. from the University of South Australia have published the research: Staining the endoplasmic reticulum in combination with antibody staining, in the Journal: (JOURNAL)
  • what: In this protocol the authors show that a commercial endoplasmic_reticulum marker ReZolve-ER can be used to visualise the endoplasmic_reticulum following standard immunofluorescence antibody labelling.
  • how: For this protocol the authors used the adherent cell line PNT2 grown in RPMI media supplemented with 10 % fetal bovine serum. For this Page 2/4 example the authors used goat anti-rabbit Alexa Fluor 647 at a . . .

     

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