Stat5b-mediated growth hormone signaling is organized by highly dynamic microtubules in hepatic cells*

HIGHLIGHTS

• who: Thu Phung-Koskas from the with PEM buffer, and soluble proteins were extracted in the same buffer supplemented with, % Triton , and the antiprotease and antiphosphatase mixture. Soluble protein extraction was followed by one rinse in Triton-free buffer. Incubation of extracted cells in PEM buffer during , min at , °C allowed the dilution-induced depolymerization of the most labile MTs. To be sure that no dynamic MT remained in extracted cells, cells were further incubated for a prolonged period of time with PEM (, h at , °C). Stable MTs that remained in permeabilized cells were subsequently depolymerized by . . .
  

   

  

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