HIGHLIGHTS
SUMMARY
Following the Cas9 restriction, the DNA was labelled for ODM experiments according to the slightly modified protocol 4 μM DNA (base_pairs, directly from the Cas9 mixture, without purification), 1.2 μM (base_pairs) λ-DNA, 2.6 μM YOYO-1 (ratio of DNA base_pairs:YOYO-1 2:1), and 260 μM netropsin (netropsin:YOYO-1 ratio 100:1) followed by incubation at 50 °C for 30 min. The reference is stretched to the experimentally measured nanometer per base_pair stretch factor determined using the reference molecule λ-DNA, the alignment allows for fluctuations of the DNA molecule (with . . .
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