HIGHLIGHTS
SUMMARY
An unstructured positively charged Ndc80 tail that precedes the CH domain is crucial for MT tip tracking, and phosphorylation of the tail or introduction of negative charges reduces the binding affinity of the Ndc80 complex to the MT lattice. The elastic properties of the Ndc80 complex in its MT-bound state, in particular its stiffness under tension, are the main subject of this work. Although in those experiments the stiffness was only determined while the Ndc80 complexes were attached to a stalled MT, here, the authors re-evaluate the same data with a novel . . .
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