HIGHLIGHTS
- who: Sachin S. Katti from the M University have published the article: Structural anatomy of Protein Kinase C C1 domain interactions with diacylglycerol and other agonists, in the Journal: NATURE COMMUNICATIONS NATURE COMMUNICATIONS of 18/Oct/2021
- how: To determine the predominant DAG-binding mode in solution the authors conducted solution NMR experiments on C1Bu03b4-DAG assembled in isotropically tumbling bicelles. For the isotopically enriched C1Bu03b4 preparations the authors used the resuspension method41 in the M9 minimal medium supplemented with 15NH4Cl and D-13C6glucose as nitrogen and carbon sources respectively. For the C1Bu03b4-DAG complex . . .
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