Structural basis for cannabinoid-induced potentiation of alpha1-glycine receptors in lipid nanodiscs

HIGHLIGHTS

• who: Arvind Kumar from the Electrophysiological recordings by two-electrode voltage-clamp (TEVC) in oocytes The pCS2-a, plasmid encoding zebrafish GlyRu03b1, for expression in Xenopus laevis was kindly provided by ProfRobert Vandenberg, University of Sydney, Australia. (Supplementary Table, ). The mutations were introduced using standard site-directed mutagenesis protocol. To linearize the DNA, the plasmid was incubated with Nde, restriction enzyme at , u00b0C for , h. The mRNA was synthesized from linearized DNA using the mMessage mMachine kit (Ambion) per instructions in the manufacturer`s manual. The RNA was then purified with RNAeasy kit (Qiagen). About, .2, ng . . .
  

   

  

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