HIGHLIGHTS
- who: Christopher M. Furze from the C)The resulting PCR product was cloned into a modified pETSUMO vector (a gift from Dr Patrick Moynihan, University of Birmingham) using the BamHI and HindIII restriction enzyme sites resulting in the construct mtr_lpqY_sumo. Targeted single-site substitutions were introduced into mtr_lpqY_sumo using the primers that are detailed in Table, with Phusion HF polymerase, and the PCR cycle (, have published the article: Structural basis of trehalose recognition by the mycobacterial LpqY-SugABC transporter, in the Journal: (JOURNAL) of October/20,/2020
- what: The authors propose that Mtb has evolved . . .
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