Structural remodelling of the carbon-phosphorus lyase machinery by a dual abc atpase

HIGHLIGHTS

• who: Su00f8ren K. Amstrup from the Cryo-EM sample preparation and analysis of Phn(GHIJKL) PhnK E Q UltrAuFoil, grids were glow discharged (, mA, s, GloQube, Quorum) immediately before dispensing , u03bcL freshly purified protein diluted in StrepTrap wash buffer to a concentration of, ., mg/mL followed by blotting (7, s) and immediate plunge-freezing in liquid ethane using a Leica plunge freezer EM GP2, adjusted to , u00b0C, % humidity and an ethane temperature of , u00b0C. Cryo-EM data was acquired at the Danish National Cryo-EM Facility (EMBION), AU node (iNANO, Aarhus University) on a Titan Krios, i . . .
  

   

  

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