Structure of the human nk cell nkr-p1:llt1 receptor:ligand complex reveals clustering in the immune synapse

HIGHLIGHTS

• who: Jan Blu00e1ha from the Protein expression and purification Stabilized H C form of the soluble , ectodomain (Gln72-Val191) was transiently expressed in HEK S GnTI- cells, as previously described The N R, R E, K A secondary interaction mode mutant LLT SIM was cloned and produced in the same way. The, type lectin-like domain of human NKR-, was produced similarly in stably transfected HEK S GnTI- cells50. Briefly, the expression construct corresponding to the extracellular CTL domain of NKR-, (Gly Ser225) was subcloned into the pOPINGGTneo plasmid (kindly provided by Prof. Ray Owens, University of . . .
  

   

  

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