Subcellular compartmentalization of activation and desensitization of responses mediated by nk2 neurokinin receptors*

HIGHLIGHTS

• who: Jean-Yves Vollmer from the cDNA Constructs, Expression, and Selection-Rat NK R cDNA (56) was provided by ProfS. Nakanishi (Kyoto University), a7- , cDNA (57) in KS vector was provided by Prof. J. P. Changeux (Institut Pasteur, Paris, France), PKCa-EGFP in pEGFP-, vector was obtained from Dr. D. Joubert (INSERM, Montpellier, France). The a , sequence was excised from the plasmid by the restriction enzymes BsrGI and XhoI leaving the a, signal sequence (NotI-BsrGI fragment) linked to the KS vector. EGFP (pEGFP-C3, CLONTECH) was mutated to introduce an additional BsrGI site (Sculptor site-directed . . .
  

   

  

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