HIGHLIGHTS
SUMMARY
Coinfection can also be demonstrated experimentally in_vivo: for example, when using a virus that was dependent on coinfection for replication, amplification of the virus was observed in the nasal passages of guinea pigs, indicating that coinfection occurred during multiple rounds of infection. To do this, the authors needed to visualise infected lesions directly, which the authors did using fluorescent reporter viruses, an approach that has been used previously to identify coinfected cells in_vitro, and which has been used to identify coinfected cells in_vivo following high-dose intranasal inoculation of mice. The ability of viruses . . .
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