Suppression of

HIGHLIGHTS

• who: Haitang Wang from the Pure Plant Kit (TIANGEN, Beijing, China)To generate Gh, overexpression and antisense transgenic cotton lines, the full-length coding sequence of Gh, was amplified using primers OE-GhGLU19-F/R. The fragments were then inserted into Xba I and BamH I sites of p, vector through standard cloning procedures [49]. p, is a binary vector, with kanamycinresistance for plant selection, that expression of gene of interest was controlled by Cauliflower mosaic virus S promoter. For the antisense construct, the ORF sequence was reversely inserted into p, vector following the same cloning procedures . . .
  

   

  

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