Suppression of mrnas encoding cd63 family tetraspanins from the carcinogenic liver fluke opisthorchis viverrini results in distinct tegument phenotypes

HIGHLIGHTS

• who: Sujittra Chaiyadet from the Experimental animals and Oviverrini. O. viverrini metacercariae were collected from naturally infected cyprinoid fish as previously described24. Fifty metacercariae were used to infect, week-old Golden Syrian hamsters (Mesocricetus auratus) by the oral route using intragastic tube feeding. Hamsters were euthanized, blood was obtained by cardiac puncture and worms were recovered from the gall bladders and bile ducts. Juvenile and adult O. viverrini worms were harvested from infected hamster livers at , weeks and , weeks, respectively. Eggs of O. viverrini were obtained from cultured worms in RPMI supplemented with, x antibiotics (PenicillinStreptomycin , µg . . .
  

   

  

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